Abstract:
Fruits are rich in antioxidants which help in
lowering incidence of degenerative diseases such as cancer,
arthritis, heart disease, inflammation and acceleration
of the ageing process. It is very important point to move
to conventional fruits because the fruits which are
highlighted as good antioxidant sources are very expensive.
This study was carried out to determine the antioxidant
activity (AC) in the formulation which was proposed by
Brand Williams, and it is based on the inhibition of the
free radical 2, 2-diphenyl-1-picrilhydrazil (DPPH) in
ethanol extract of the samples. According to the results
water melon (Citrullus lanatus) got the highest EC50
value (16.381± 0.698mg/ml) while star fruit (Averrhoa
carambola), got the lowest EC50 value (0.073± 0.011mg/
ml) among these selected fruits. It means that water melon
(C. lanatus) has the lowest antioxidant activity while star
fruit (A. carambola) has the highest antioxidant activity
among these selected fruits. None of the samples showed
more or less similar EC50 for standard L-Ascorbic acid
(0.005±0.001mg/mL). Among these selected vegetables,
star fruit (A. carambola) and bilin (Averrhoa bilimbi)
are not significantly different from standard sample
that was L-Ascorbic acid. Except these two fruits, all
the other selected fruit samples significantly differ from
L-Ascorbic acid. avocado (Persea americana) significantly
differs from the other fruit samples. So, that EC50 value
is reducing significantly (p<0.05) as water melon (C.
lanatus) 16.381± 0.698mg/mL > banana (Musa) 4.308±
0.265mg/mL > papaya (Carica papaya) 4.290±0.388mg/mL
> pineapple (Ananas comosus) 1.942±0.052mg/mL > egg
fruit (Pouteria campechiana) 1.712±0.030mg/mL > mango
(Mangifera indica) 1.180±0.128mg/mL > duriyan (Durio
sp.) 1.157±0.112mg/mL > avacado (Persea americana)
0.795±0.083mg/mL > bilin (A. bilimbi) 0.095±0.011mg/mL
> star fruit (A. carambola) 0.073±0.011mg/mL. Conversely
DPPH antioxidant activity increased significantly in
ascending order. Considering these results, the local fruits
also contain high antioxidant activities.