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Effect of selected polyols and salts on stability of xylanase produced by bacillus pumilus

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dc.contributor.author Subajini, M.
dc.contributor.author Balakumar, S.
dc.contributor.author Vasanthy, A.
dc.date.accessioned 2021-07-16T08:22:05Z
dc.date.accessioned 2022-07-07T07:21:17Z
dc.date.available 2021-07-16T08:22:05Z
dc.date.available 2022-07-07T07:21:17Z
dc.date.issued 2014
dc.identifier.issn 2279-1922
dc.identifier.uri http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/3672
dc.description.abstract The objective of this study is to improve the stability of an alkaline xylanase with selected polyols and salts, which was produced by a locally isolated alkalo-thermophilic Bacillus pumilus. Xylanases with higher stability are used for the application in paper and pulp industry for bio bleaching. In the absences of additives at 55˚C, the xylanase retained 38(±1.0)% of its initial activity at 30min and retained 45.6 (±0.76) and 20.6(±0.84)% of its initial activity at pH 8.0 and 9.0 respectively. Xylanase which contained polyols such as 10mM Poly Ethylene Glycol (PEG)-8000, 1M glycerol and 2M sorbitol the enzyme retained 24.0(±0.34), 19.0(±0.84) and 53.8(±0.57)% of its initial activity respectively at 60min. Addition of 10mM NaCl lost all of its activity while the enzyme retained 88.4(±0.18)% of its initial activity in the presence of 10mMCaCl2. Sorbitol and CaCl2 were the best additives among the polyols and salts respectively. CaCl2 and sorbitol of different concentrations were studied and the halflives of the xylanase in presences of 10mM CaCl2 and 2M sorbitol were 302 and 63min respectively. When both 10mM CaCl2 and 2M sorbitol were used together, the enzyme retained more (95%) of its initial activity at 60min than that in presence of 10mM CaCl2 (88%) and 2M sorbitol (53%) individually. At 55˚C half-life of the xylanase in presence of CaCl2, sorbitol and CaCl2 & sorbitol were 18, 47 and 552min respectively. The enzyme contained both CaCl2 & sorbitol helped to retain 95, 88 and 18% of the initial activity at 55, 60 and 65oC respectively; while at 70oC it lost all of its activity at 120min. Xylanase from B.pumilus was stable at 60oC for 2h with both CaCl2 & sorbitol. en_US
dc.language.iso en en_US
dc.publisher University of Jaffna en_US
dc.subject Half-life en_US
dc.subject Polyols en_US
dc.subject Stability en_US
dc.subject Salts en_US
dc.subject Xylanase en_US
dc.title Effect of selected polyols and salts on stability of xylanase produced by bacillus pumilus en_US
dc.type Article en_US


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