Abstract:
IQGAP related proteins are found in a number of organisms
including human and yeast. Iqg1p is a cytoskeletal scaffolding protein found
in Saccharomyces cerevisiae. It has critical role in cell division and disruption of
the protein which results in growth defects. The IQ motif of Iqg1p has been
reported to bind to EF hand proteins such as Mlc1p in vivo to co-ordinate
various cellular events. It has not yet been known which of the IQ motif
binds to the Mlc1p in vivo / in vitro. This computational approach identifies
for the first time that the seventh and ninth IQ motifs have high probability
for binding with Mlc1p followed by the eighth IQ motif. However the first IQ
motif has less probability for binding with Mlc1p. Further, it is noted that
this work is supported by the in vivo work of Terrak et al. (2003). The
observation raises a possibility that EF hand target sequences are larger than
previously reported consensus sequences.
