Abstract:
Addressing global malnutrition through the biofortification of staple crops is essential
for improving public health and nutritional security. Sweet corn (Zea mays), a widely
consumed vegetable, offers a unique opportunity to enhance dietary intake of essential
nutrients, particularly beta carotene, a precursor to vitamin A. This study focuses on
evaluating sweet corn inbreds with elevated beta carotene content to identify optimal
parental lines for developing superior hybrids. The sweet corn lines developed in Tamil
Nadu Agricultural University were utilized. Four sweet corn inbred lines, DBT 15, DBT
18, DBT 23, and DBT 25, were selected for their high beta carotene and sugar content
during Summer 2024. The total sugar content and beta carotene levels were quantified
in the 24th day after flowering, using the anthrone method and High-Performance Liquid
Chromatography (HPLC), respectively. Additionally, marker analysis was performed to
confirm the presence of the crtRB1 gene, which is crucial for beta carotene biosynthesis.
The evaluations revealed the following results for total sugar content (%): DBT 15 with
15.26%, DBT 18 with 14.19%, DBT 23 with 14.9%, and DBT 25 with 15.2%. The beta
carotene concentrations (μg/g) were: DBT 15 with 8.369, DBT 18 with 8.381, DBT 23
with 8.389, and DBT 25 with 7.894. Among these lines, DBT 15 and DBT 25 exhibited
the highest sugar content, while DBT 18 and DBT 23 had the highest beta carotene
levels. This research highlights the potential of these inbred lines as valuable parents
for breeding nutritionally enhanced sweet corn hybrids. By identifying and utilizing
superior parental lines, future hybrids can be developed to combine high beta carotene
and sugar content, addressing both nutritional deficiencies and consumer preferences.
Our findings contribute to the broader effort of food biofortification, promoting better
health through enriched diets.
