Abstract:
Introduction: Staining plays a pivotal role in the morphological evolution of tissue
samples in histopathology. The combination of Hematoxylin and Eosin is the most
common stain utilized for tissue staining. Hematoxylin, a nuclear stain, is a natural dye,
whereas eosin is a synthetic dye. The use of synthetic dyes raises concerns about their
harmful effects on both laboratory workers and the environment. Consequently, finding an
alternative eco-friendly natural dye to replace eosin is inevitable to improve the laboratory
diagnosis in histopathology.
Objective: To evaluate the staining efficacy of a dye isolated from Tectona grandis Linn.
on tissue samples.
Methodology: Young leaves of T.grandis Linn. were collected and washed with tap water.
The cleaned leaves were subjected to drying in an oven at 40°C and ground into fine
powder. The extract was obtained by combining the dry plant material with 96% ethanol at
60°C for 4 hours and allowed at 25°C for12 hours. The mixture was filtered through
Whatman paper followed by centrifugation at 5000 rpm for 15 minutes. The collected
supernatant was removed using a Rotary evaporator and redissolved in absolute methanol
to a final concentration of 20mg/ml. The staining ability of the dye was evaluated on 5 μm
thick sections of human appendix tissues.
Results: The findings demonstrated that the dye isolated from young leaves of Teak
exhibits a staining potential on appendix tissues. Particularly, the staining ability of T.
grandis Linn derived dye at a concentration of 20 mg/ml on tissue samples was comparable
to those stained by Eosin.
Conclusion: A dye extracted from T. grandis Linn. exhibits notable staining potential on
histological tissues which could be utilized as an alternative eco-friendly natural stain for
histological staining.
