Abstract:
The amount of commercially available food items containing genetically modified
organisms have increased in recent years. Regulation for the labeling of Genetically
Modified food is required in most of the countries. Sri Lanka is one of the countries having
labeling policy for food products containing Genetically Modified Organisms. For this
purpose, reliable and accurate detection methods are necessary. The Real time-PCR is the
most reliable detection method to detect Genetically Modified Organisms. The aim of this
study is to detect genetically modified food items in market using Real time-PCR assay. The
CTAB method and Mericon food kit methods were used for the extraction of DNA from the
market food products. The Mericon GMO detection kit was used to perform the Real time PCR assays. The screening of the products was based on detection of the Cauliflower mosaic
virus 35S promoter. According to the results, all of the extracted samples from CTAB
method were positive while one of the four extracted samples from Mericon food kit was
positive. The false positive results obtained from the CTAB extraction could be due to the
infection of cauliflower mosaic virus in the food samples. However, the ratio of positive
samples decreased after performing Mericon kit method. The cauliflower mosaic virus
contamination could have removed by the high purification of Mericon food kit. According
to the results, out of four samples one sample imported from U.S.A. was positive for CaMV
35S promoter, indicating that it is a GMO. With further evaluation, this method could be
used in future testing for GMO.
