Abstract:
Inorganic fertilizer usage leads to economic losses and environmental issues. Limited knowledge
and availability of organic fertilizers is one of the reasons for excessive inorganic fertilizer usage.
Though phosphorus and potassium are rich in plant residues, their nitrogen content is poor.
Azolla (Az) and Spirulina (Sp) are nitrogen-rich sources grown in water, which is a scarce
resource. In this background, a study was conducted aiming to formulate low-cost medium using
wastewater to produce Spirulina and Azolla and analyze different organic nutrient sources,
namely, Palmyrah leaf (Pl), Coconut leaf (Co), Banana pseudostem (Bp), Az and Sp. Biomass yield
of Spirulina was assessed with different combinations of wastewater and standard Zarrouk’s
medium (Zm) with two replicates and six treatments, namely, T1- 100% Zm, T2- 50% Zm, T3 -
50% Zm + 50% Parboiled effluent, T4 - 50% Zm + 50% Reverse osmosis wastewater (ROw), T5-
50% Zm + 50% Seawater, T6 - 50% ROw. Azolla was assessed with different combinations of
wastewater and freshwater with two replicates and nine treatments, namely, T1 - Kitchen
wastewater (Kw) + Triple superphosphate (TSP), T2- Tap water (Tw) +TSP, T3- ROw + TSP, T4 -
Kw + Cow dung (Cd), T5 - Tw +Cd, T6 - ROw + Cd, T7 -Kw, T8 - Tw, T9 - ROw in completely
randomized design (CRD). Macronutrient (carbon, nitrogen, phosphorous, potassium, calcium,
and magnesium) content of organic sources Az, Sp, Co, Pl, and Bp were analyzed with three
replicates in CRD. The recorded data were statistically analyzed using ANOVA, and mean
separation was done using Duncan Multiple Range Test. Results of nutrient analysis of organic
sources indicated that significantly higher potassium (19.56 mg/kg), phosphorous (6.17 mg/kg),
nitrogen (115.03 mg/kg), carbon (832.50 mg/kg), calcium (10861 mg/kg) and magnesium
(1947.2 mg/kg) were found in Banana pseudostem, Banana pseudostem, Spirulina, Palmyra leaf,
Azolla, and Azolla, respectively. By substituting 50% Zm with parboiled effluent (3.02 g/L) or
ROw (2.8 g/L) gave significantly higher and comparable Spirulina dry biomass respectively to
that of 100 % Zm (2.809 g/L). Significantly higher dry biomass yield was obtained in Azolla
when Kw or ROw was used instead of freshwater. Results therefore indicated that the tested
underutilized organic sources and nitrogen sources grown in wastewater are potential plant
nutrient sources.
