Abstract:
Antimicrobial herbs can be used individually or in
combination to prepare mouth wash which is healthier
and safer than the synthetic ones. In this study a new
“Accmus” herbal mouth wash was prepared and its
antibacterial properties were evaluated. Alcoholic, boiled
alcoholic and aqueous extracts of “Accmus” mouth wash
were prepared from the bark of Acacia arabica, Acacia
speciosa and root of Calamus rotang in combination by
tincture and hot extract methods respectively. Alcohol
content and pH were also determined. Antibacterial
properties of the above extracts were also studied against
Staphylococcus aureus, Bacillus sp of Gram (+)ve and
Pseudomonas aeruginosa, Klebsiella sp of Gram (-) ve
in vitro by using agar well diffusion method. This study
showed that the alcohol content and pH of mouth wash
preparations were in acceptable levels. Aqueous extract
exhibited better antibacterial activity compared with
alcoholic extract and had maximum sensitivity towards
Bacillus sp and low towards Klebsiella sp. Staphylo coccus aureus was only inhibited by all preparations of
mouth wash. So the hot extraction method was efficient
than the alcoholic extraction and this could be
recommended with antibacterial properties rather than
the alcoholic extract of mouth wash. Further study is
needed for further purification and characterization of
active constituents from various solvent extracts of mouth
wash against oral diseases.
