Isolation of a cellulase producing Bacillus cereus from cow dung and determination of the kinetic properties of the crude enzyme.

Abstract

The objective of the study was to isolate a thermostable alkaline cellulase producing bacterial strain from diverse natural sources such as goat excreta, cow dung, tropical soil, and organic matter. Opened hot cellulose agar plates were transferred on to the selective carboxy methyl cellulose agar media and incubated for 2 days at 37 o bacteria. The bacteria isolated were screened for cellulolytic activity using serial dilution and pour-plate method after which they were characterised. The bacterial isolate with the highest biochemical and cultural analysis, the strain from cow dung Bacillus sp. Pure culture of this bacterial using automated sequencer and sequence similarity search was Bacillus cereus Bacillus cereus showed zero order kinetics for 20 minutes. The optimum pH for the crude cellulase from Bacillus cereus was 9.0 at 45o and the temperature optimum was 45o constant for the cellulase from this isolate to soluble cellulose -1L and Vmax was 3.32 µmol mL-1 min-1 at pH 9.0 and at 45 o than an hour at 45o good candidate for industrial applications