Abstract:
The objective of the study was to isolate a
thermostable alkaline cellulase producing bacterial strain from
diverse natural sources such as goat excreta, cow dung, tropical
soil, and organic matter. Opened hot cellulose agar plates were transferred on to the selective carboxy methyl cellulose
agar media and incubated for 2 days at 37 o
bacteria. The bacteria isolated were screened for cellulolytic
activity using serial dilution and pour-plate method after which
they were characterised. The bacterial isolate with the highest
biochemical and cultural analysis, the strain from cow dung
Bacillus sp. Pure culture of this bacterial
using automated sequencer and sequence similarity search was
Bacillus cereus
Bacillus cereus
showed zero order kinetics for 20 minutes. The optimum pH
for the crude cellulase from Bacillus cereus was 9.0 at 45o
and the temperature optimum was 45o
constant for the cellulase from this isolate to soluble cellulose -1L and Vmax
was 3.32 µmol mL-1 min-1 at pH 9.0
and at 45 o
than an hour at 45o
good candidate for industrial applications
