Abstract:
Lime is a widely produced, economical fruit that is nutritious and healthy. Processing
lime produces large amounts of waste, including peel, pulp, pomace, and seeds, and improper
disposal can lead to environmental pollution. This study aims to investigate the antioxidant
properties of different portions of lime waste as a functional ingredient, helping to valorize by-
products and mitigate environmental pollution. Lime waste was obtained and separated into peel
(P1), pulp (P2), and a mixture of both (P3). The samples were dried at 60 °C for 12 hours and
ground into flour (particle size 150 μm). Methanolic extracts were prepared using 5 g of each
waste and 50 mL of methanol, and the total phenolic content (TPC), total flavonoid content
(TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic
acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays of the lime waste extracts
were analyzed in duplicate. Overall, peels had significantly higher polyphenols (27.35 ± 0.06 mg
GAE g-1), while pulp (17.526 ± 0.28 mg GAE g-1) and the mixture of peel and pulp (19.256 ±
0.08 mg GAE g-1) had significantly lower polyphenol contents (P<0.05). A similar pattern of
high results was observed for the antioxidant potential of lime peel among the waste portions in
TFC (0.068 ± 0.01 mg QE g-1) and in the IC50 values of DPPH (0.200 mg mL-1), ABTS (0.069
mg mL-1), and FRAP (0.154 mg mL-1) assays. In conclusion, polyphenols, flavonoids, and
other antioxidants are present in lime waste, with levels significantly higher in lime peel,
intermediate in the mixture of both, and lowest in pulp. Hence, lime waste contains beneficial
compounds, making it a functional raw material that helps valorize by-products and mitigate
environmental pollution.
