Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification(AI-LAMP) for Rapid Detection of SARS-CoV-2

Rohaim, M.A.; Clayton, E.; Sahin, I.; Julianne, V.; Khalifa, M.E.; Al-Natour, M.Q.; Bayoumi, M.; Poirier, M.C.; Branavan, M.; Mukunthan, T.; Chaudhry, N.S.; Ravinder, S.; Amy, B.; Peter, B.; Hacking, W.; Botham, J.; Boyce, J.; Wilkinson, H.; Williams, C.; Whittingham-Dowd, J.; Shaw, E.; Matt Hodges; Lisa Butler; Bates, M.D.; Roberto La, R.; Balachandran, V.; Anil, F.; Munir, M.

dc.contributor.author	Rohaim, M.A.
dc.contributor.author	Clayton, E.
dc.contributor.author	Sahin, I.
dc.contributor.author	Julianne, V.
dc.contributor.author	Khalifa, M.E.
dc.contributor.author	Al-Natour, M.Q.
dc.contributor.author	Bayoumi, M.
dc.contributor.author	Poirier, M.C.
dc.contributor.author	Branavan, M.
dc.contributor.author	Mukunthan, T.
dc.contributor.author	Chaudhry, N.S.
dc.contributor.author	Ravinder, S.
dc.contributor.author	Amy, B.
dc.contributor.author	Peter, B.
dc.contributor.author	Hacking, W.
dc.contributor.author	Botham, J.
dc.contributor.author	Boyce, J.
dc.contributor.author	Wilkinson, H.
dc.contributor.author	Williams, C.
dc.contributor.author	Whittingham-Dowd, J.
dc.contributor.author	Shaw, E.
dc.contributor.author	Matt Hodges
dc.contributor.author	Lisa Butler
dc.contributor.author	Bates, M.D.
dc.contributor.author	Roberto La, R.
dc.contributor.author	Balachandran, V.
dc.contributor.author	Anil, F.
dc.contributor.author	Munir, M.
dc.date.accessioned	2023-12-29T08:15:35Z
dc.date.available	2023-12-29T08:15:35Z
dc.date.issued	2020
dc.identifier.uri	http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/10023
dc.description.abstract	Untilvaccinesandeffectivetherapeuticsbecomeavailable,thepracticalsolutiontotransit safelyoutofthecurrentcoronavirusdisease19(CoVID-19)lockdownmayincludetheimplementation ofaneffectivetesting,tracingandtrackingsystem.However,thisrequiresareliableandclinically validateddiagnosticplatformforthesensitiveandspecificidentificationofSARS-CoV-2.Here, wereportonthedevelopmentofadenovo,high-resolutionandcomparativegenomicsguided reverse-transcribedloop-mediatedisothermalamplification(LAMP)assay.Tofurtherenhancethe assayperformanceandtoremoveanysubjectivityassociatedwithoperatorinterpretationofresults, weengineeredanovelhand-heldsmartdiagnosticdevice.Therobustdiagnosticdevicewasfurther furnishedwithautomatedimageacquisitionandprocessingalgorithmsandthecollateddatawas processedthroughartificialintelligence(AI)pipelinestofurtherreducetheassayruntimeandthe subjectivityofthecolorimetricLAMPdetection.ThisadvancedAIalgorithm-implementedLAMP (ai-LAMP)assay,targetingtheRNA-dependentRNApolymerasegene,showedhighanalytical sensitivityandspecificityforSARS-CoV-2.Atotalof~200coronavirusdisease(CoVID-19)-suspected NHSpatientsamplesweretestedusingtheplatformanditwasshowntobereliable,highlyspecific andsignificantlymoresensitivethanthecurrentgoldstandardqRT-PCR.Therefore,thissystemcould provideanefficientandcost-effectiveplatformtodetectSARS-CoV-2inresource-limitedlaboratories.	en_US
dc.language.iso	en	en_US
dc.publisher	MDPI	en_US
dc.subject	SARS-CoV-2	en_US
dc.subject	Diagnosis	en_US
dc.subject	LAMP	en_US
dc.subject	Point of care	en_US
dc.subject	Artificial intelligence	en_US
dc.title	Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification(AI-LAMP) for Rapid Detection of SARS-CoV-2	en_US
dc.type	Article	en_US
dc.identifier.doi	doi:10.3390/v12090972	en_US