Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification(AI-LAMP) for Rapid Detection of SARS-CoV-2

Rohaim, M.A.; Clayton, E.; Sahin, I.; Julianne, V.; Khalifa, M.E.; Al-Natour, M.Q.; Bayoumi, M.; Poirier, M.C.; Branavan, M.; Mukunthan, T.; Chaudhry, N.S.; Ravinder, S.; Amy, B.; Peter, B.; Hacking, W.; Botham, J.; Boyce, J.; Wilkinson, H.; Williams, C.; Whittingham-Dowd, J.; Shaw, E.; Matt Hodges; Lisa Butler; Bates, M.D.; Roberto La, R.; Balachandran, V.; Anil, F.; Munir, M.

Artificial Intelligence-Assisted Loop Mediated Isothermal Amplification(AI-LAMP) for Rapid Detection of SARS-CoV-2

Rohaim, M.A.; Clayton, E.; Sahin, I.; Julianne, V.; Khalifa, M.E.; Al-Natour, M.Q.; Bayoumi, M.; Poirier, M.C.; Branavan, M.; Mukunthan, T.; Chaudhry, N.S.; Ravinder, S.; Amy, B.; Peter, B.; Hacking, W.; Botham, J.; Boyce, J.; Wilkinson, H.; Williams, C.; Whittingham-Dowd, J.; Shaw, E.; Matt Hodges; Lisa Butler; Bates, M.D.; Roberto La, R.; Balachandran, V.; Anil, F.; Munir, M.

URI: http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/10023

Date: 2020

Abstract:

Untilvaccinesandeffectivetherapeuticsbecomeavailable,thepracticalsolutiontotransit safelyoutofthecurrentcoronavirusdisease19(CoVID-19)lockdownmayincludetheimplementation ofaneffectivetesting,tracingandtrackingsystem.However,thisrequiresareliableandclinically validateddiagnosticplatformforthesensitiveandspecificidentificationofSARS-CoV-2.Here, wereportonthedevelopmentofadenovo,high-resolutionandcomparativegenomicsguided reverse-transcribedloop-mediatedisothermalamplification(LAMP)assay.Tofurtherenhancethe assayperformanceandtoremoveanysubjectivityassociatedwithoperatorinterpretationofresults, weengineeredanovelhand-heldsmartdiagnosticdevice.Therobustdiagnosticdevicewasfurther furnishedwithautomatedimageacquisitionandprocessingalgorithmsandthecollateddatawas processedthroughartificialintelligence(AI)pipelinestofurtherreducetheassayruntimeandthe subjectivityofthecolorimetricLAMPdetection.ThisadvancedAIalgorithm-implementedLAMP (ai-LAMP)assay,targetingtheRNA-dependentRNApolymerasegene,showedhighanalytical sensitivityandspecificityforSARS-CoV-2.Atotalof~200coronavirusdisease(CoVID-19)-suspected NHSpatientsamplesweretestedusingtheplatformanditwasshowntobereliable,highlyspecific andsignificantlymoresensitivethanthecurrentgoldstandardqRT-PCR.Therefore,thissystemcould provideanefficientandcost-effectiveplatformtodetectSARS-CoV-2inresource-limitedlaboratories.

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