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Comparative characterization of two cxcl8 homologs in oplegnathus fasciatus: genomic, transcriptional and functional analyses

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dc.contributor.author Umasuthan, N.
dc.contributor.author Bathige, S.D.N.K.
dc.contributor.author Thulasitha, W.S.
dc.contributor.author Oh, M.
dc.contributor.author Lee, J.
dc.date.accessioned 2021-11-24T06:24:21Z
dc.date.accessioned 2022-07-11T09:06:02Z
dc.date.available 2021-11-24T06:24:21Z
dc.date.available 2022-07-11T09:06:02Z
dc.date.issued 2020
dc.identifier.uri http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/4250
dc.description.abstract CXCL8 (interleukin-8, IL-8) is a CXC family chemokine that recruits specific target cells and mediates inflammation and wound healing. This study reports the identification and characterization of two cxcl8 homologs from rock bream, Oplegnathus fasciatus. Investigation of molecular signature, homology, phylogeny, and gene structure suggested that they belonged to lineages 1 (L1) and 3 (L3), and designated Ofcxcl8-L1 and Ofcxcl8-L3. While Ofcxcl8-L1 and Ofcxcl8-L3 revealed quadripartite and tripartite organization, in place of the mammalian ELR (Glu-Leu-Arg) motif, their peptides harbored EMH (Glu-Met-His) and NSH (Asn-Ser-His) motifs, respectively. Transcripts of Ofcxcl8s were constitutively detected by Quantitative Real-Time PCR (qPCR) in 11 tissues examined, however, at different levels. Ofcxcl8-L1 transcript robustly responded to treatments with stimulants, such as flagellin, concanavalin A, lipopolysaccharide, and poly(I:C), and pathogens, including Edwardsiella tarda, Streptococcus iniae, and rock bream iridovirus, when compared with Ofcxcl8-L3 mRNA. The differences in the putative promoter features may partly explain the differential transcriptional modulation of Ofcxcl8s. Purified recombinant OfCXCL8 (rOfCXCL8) proteins were used in in vitro chemotaxis and proliferation assays. Despite the lack of ELR motif, both rOfCXCL8s exhibited leukocyte chemotactic and proliferative functions, where the potency of rOfCXCL8-L1 was robust and significant compared to that of rOfCXCL8-L3. The results, taken together, are indicative of the crucial importance of Ofcxcl8s in inflammatory responses and immunoregulatory roles in rock bream immunity. en_US
dc.language.iso en en_US
dc.publisher University of Jaffna en_US
dc.subject CXC chemokine en_US
dc.subject CXCL8 en_US
dc.subject Interleukin-8 (il-8) en_US
dc.subject Molecular profiling en_US
dc.subject Genomic sequence arrangement en_US
dc.subject Promoter prediction analyses en_US
dc.subject Transcriptional expression en_US
dc.subject Chemotaxis en_US
dc.subject Leukocyte proliferation en_US
dc.title Comparative characterization of two cxcl8 homologs in oplegnathus fasciatus: genomic, transcriptional and functional analyses en_US
dc.type Article en_US


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