http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/1 Thu, 09 Apr 2026 17:00:10 GMT 2026-04-09T17:00:10Z http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12457 Title: Anti-Bacterial Potential of Siddha Herbo-Mineral Formulation Linga Chenduram: An In-Vitro Study Authors: Soruban, T.; Sathiyaseelan, V. Abstract: Many existing antibiotics have limitations regarding their effectiveness against various pathogens and often cause adverse effects. Overuse of these antibiotics has led to the emergence of drug-resistant microorganisms. The Siddha system of medicine offers promising potential for combating these resistant pathogens. Linga Chenduram (LC), a traditional herbo-mineral preparation mentioned in the ancient Siddha text Anuboga Vaithiya Navanitham, was the focus of this study. The aim of this study was to screen the anti – bacterial potential of Siddha herbo-mineral formulation LC. Anti-bacterial activity of the sample was tested for E.coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) to determine the diameter of inhibition zone (DIZ), minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC). The study results demonstrate that a concentration of 1000 μg/mL of LC effectively inhibited the growth of all tested organisms. The minimum bactericidal concentration (MBC) was determined to be 250 μg/mL. At this concentration, the remaining colony counts were as follows: E. coli (285 colonies, equivalent to 14.2 x 10³ CFU/mL), Pseudomonas aeruginosa (96 colonies, equivalent to 4.8 x 10³ CFU/mL), and Staphylococcus aureus (33 colonies, equivalent to 1.65 x 10³ CFU/mL). The minimum inhibitory concentration (MIC) at which 50% of the bacteria were inhibited (MIC50) was 405.584 μg/mL, 459.61 μg/mL, and 515.575 μg/mL for E. coli, Pseudomonas aeruginosa, and Staphylococcus aureus, respectively. Based on these results, it can be concluded that Linga Chenduram (LC) exhibits promising antibacterial activity against E. coli, P. aeruginosa, and S. aureus. This suggests its potential as a natural alternative or adjunct therapy for infections caused by these pathogens Wed, 01 Jan 2025 00:00:00 GMT http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12457 2025-01-01T00:00:00Z http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12456 Title: Comparative Phytochemical and Inorganic Ion Analysis of Vellai Vengaya Kulligai: A Siddha Herbo-Mineral Preparation using two ingredient variants Authors: Prasad, V.M.; Sathiyaseelan, V.; Velauthamurty, K. Abstract: With the increasing global recognition of traditional and indigenous medical systems, there is a growing emphasis on the need to standardize and scientifically evaluate the quality of traditional formulations. Vellai Vengaya Kuligai (VVK) is a herbo-mineral formulation in Siddha medicine traditionally used for managing conditions such as Mantham (indigestion), Kazhichal (diarrhea), and Vaayu (pain). Comprising 23 ingredients primarily herbal, with detoxified borax and rock salt VVK holds a prominent place in Siddha therapeutics. Although the term “Vellai Vengayam” suggests the use of white onion, classical Siddha literature indicates the substitution of garlic (Ulli) in its preparation. This study investigates the phytochemical profile of VVK prepared using two methods, one with white onion and the other with garlic and compares their constituents. While white onion is known to contain alkaloids, tannins, phenols, and terpenes, the corresponding VVK tablets were found to have alkaloids, flavonoids, saponins, and terpenes. In contrast, garlic contains alkaloids, phenols, tannins, and saponins, and the tablets prepared with garlic exhibited a broader spectrum, including alkaloids, flavonoids, phenols, tannins, saponins, terpenes, and trace inorganic elements such as iron, nickel, and nitrate. The enhanced phytochemical content observed in the garlic-based tablets suggests greater therapeutic potential as described in classical Siddha texts. This preliminary comparative analysis provides a scientific foundation for understanding the compositional differences arising from variation in preparation methods Wed, 01 Jan 2025 00:00:00 GMT http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12456 2025-01-01T00:00:00Z http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12455 Title: Standardization of Keezhanelli Chooranam (KNC): A Single - Herbal Siddha Formulation Authors: Soruban, T.; Sathiyaseelan, V. Abstract: Standardization of herbal formulations is essential to ensure the quality, safety, and efficacy of traditional medicines. This study aimed to establish standardization parameters for Keezhanelli Chooranam (KNC), a Siddha formulation prepared from Phyllanthus amarus, traditionally used in the management of Madhumegam (diabetes mellitus). KNC was prepared according to classical Siddha methods and analyzed using modern techniques in accordance with AYUSH guidelines. Physico- chemical evaluation revealed a brownish, moderately fine powder with characteristic odour, particle size of 94.67 ± 25.5 μm, pH 6.6, and good solubility in water, ethanol, and DMSO. Loss on drying (5.467 ± 0.30%), total ash (0.31 ± 0.026%), extractive values, and absence of acid-insoluble ash were within acceptable limits. ICP-OES analysis confirmed the absence of toxic heavy metals. HPTLC profiling showed multiple phytochemical peaks with Rf values ranging from 0.01 to 0.66. Microbial studies confirmed sterility of the formulation. The results indicate that KNC meets standard quality and safety requirements, supporting its suitability for further pharmacological and clinical evaluation. Wed, 01 Jan 2025 00:00:00 GMT http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12455 2025-01-01T00:00:00Z http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12454 Title: The Study on Siddha Herbal Formulation Keezhanelli Chooranam for the Treatment of Type 2 Diabetic (Madhumegam): A Review Authors: Soruban, T.; Sathiyaseelan, V. Abstract: Diabetes Mellitus (DM) is a common metabolic disorder characterized by high blood glucose levels and symptoms such as polyuria, polydipsia, and polyphagia. Type 2 Diabetes Mellitus (T2DM) is the most prevalent form worldwide and is associated with serious complications including cardiovascular disease, neuropathy, nephropathy, and retinopathy. In Siddha medicine, T2DM can be correlated with Madhumegam. Keezhanelli Chooranam (KNC), prepared from Phyllanthus amarus, is traditionally used in Siddha practice for the management of Madhumegam. This review aims to collect classical Siddha literature evidence and scientific research findings related to KNC, especially its antidiabetic property. Literature from authentic Siddha texts, pharmacopoeias, research articles, and electronic databases were reviewed. Phytochemical studies show that Phyllanthus amarus contains lignans, flavonoids, tannins, alkaloids, and triterpenes, which possess antioxidant, anti-inflammatory, antidiabetic, and anticancer properties. Animal studies suggest significant antidiabetic effects, although human studies show mixed results. Overall, Keezhanelli Chooranam has promising therapeutic potential, but more well-designed clinical trials are required to confirm its safety and effectiveness in humans. Thu, 01 Jan 2026 00:00:00 GMT http://repo.lib.jfn.ac.lk/ujrr/handle/123456789/12454 2026-01-01T00:00:00Z